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Quality and testing

Janoshik Peptide Testing: What a Report Shows

What a Janoshik peptide test report shows, which fields come from the lab and which from the client, and how to verify a report with its task number and key.

Published · 9 min read · Anhydrolabs

Janoshik is an independent analytical laboratory that tests samples sent in by its customers, including research peptides, and issues a test report for each sample. A standard peptide report states the compound identified, the measured amount per vial in milligrams, and HPLC purity. Every report carries a task number and a unique key, and entering both on Janoshik's verification page returns the lab's own copy, which is how a reader confirms that a report is genuine and unaltered.

This post explains what a Janoshik report contains, which parts are measurements and which are details supplied by whoever sent the sample, how to verify one step by step, and what a verified report does and does not establish.

What Janoshik is

Janoshik Analytical describes itself as an independent testing laboratory. It accepts samples from customers worldwide, analyzes them, and delivers a digital report by email and through a customer account. Its catalog covers peptides and a range of other compounds, and it offers additional tests on request.

Three features of how it works matter when you read one of its reports:

  • The customer chooses the sample. A report describes whatever physical sample the customer sent. The lab's own FAQ is explicit that it cannot issue a report without receiving a sample.
  • The customer supplies the labels. The name of the sample, the client name, the stated manufacturer and the batch are details the customer provides with the order.
  • Reports can be made public. Janoshik publishes a searchable list of public reports, with the note that it does not endorse, sponsor or have any affiliation with the companies, organizations or products listed.

That last point is worth taking at face value. A Janoshik report is a record of one analysis. It is not a certification of a supplier, a product line or a brand.

What a standard peptide test measures

Janoshik's catalog page for a single-peptide analysis, for example its BPC-157 test, describes the standard test as "ID, amount and purity analysis", by HPLC. For a lyophilized sample the stated format is milligrams per vial and peptide purity, with an alternative format that expresses purity on a mass-per-mass basis. The minimum sample for a lyophilized product is one whole unopened vial.

MeasurementWhat it answersStandard or add-on
IdentityIs the named compound present?Standard
Amount (mg per vial)How much of the compound is in this vial?Standard
Purity (%)Of the related material detected, how much is the target?Standard
TFA analysisHow much trifluoroacetate counter-ion is present?Add-on
LC-MS screening for peptide contaminationIs another peptide present that should not be?Add-on
CHNS mass reportElemental composition by massAdd-on
EndotoxinHow much bacterial endotoxin is present?Add-on
SterilityDo bacteria or fungi grow from the sample?Add-on
Heavy metalsArsenic, cadmium, lead, mercuryAdd-on
Variance testingDo several vials of the same batch agree?Add-on

The practical consequence is simple. A report shows only what was ordered. If the findings table lists an amount and a purity, then endotoxin, sterility, counter-ion content and cross-contamination were not measured on that sample, and the report says nothing about them. Absence of a line is absence of a test.

Anatomy of a report

A Janoshik test report is a single page with a fixed layout. The fields below appear on published reports, top to bottom.

FieldWhat it containsWho supplies it
Task numberThe lab's reference number for the analysis, shown with a #Lab
Testing orderedDate the order was placedLab
Sample receivedDate the sample arrived at the labLab
ClientName of the customer who commissioned the testCustomer
SampleFree-text name of the sample, as entered by the customerCustomer
ManufacturerStated maker of the sampleCustomer
BatchBatch or lot designationCustomer
Sample descriptionNotes on the sample, such as whether photos are includedVaries
Tests requestedThe analyses orderedOrder
Findings tableMeasured amount per vial and purity, plus any add-onsLab
CommentsAny note from the analystLab
Analysis conductedDate the analysis was performedLab
Signature and QR codeAnalyst signature; a code linking to the lab's copyLab
Unique keyA code printed at the foot, used for verificationLab

Read the report in two layers. The dates, the findings table and the verification details are the laboratory's own. The client, sample name, manufacturer and batch are descriptions the customer gave the lab. The lab measured the vial it received. It did not, and could not, establish from the analysis alone that the vial came from the manufacturer or batch written above the numbers.

How to verify a Janoshik report

Anyone can make a PDF that looks like a laboratory report. Verification is what separates the lab's record from a copy that has been edited or invented.

  1. Find the task number. It is near the top of the report, next to the heading "Task Number", shown with a # sign.
  2. Find the unique key. It is printed at the foot of the report, under a line directing readers to verify the test with the key.
  3. Open the verification page yourself. Type janoshik.com into the browser and use the Verify link in the site menu, rather than following a link supplied with the report. The page asks for two things: the task number and the unique key.
  4. Enter both codes. A genuine pair returns the report as the lab holds it.
  5. Compare field by field. Check the task number, every date, the sample name, the batch, the tests requested and each figure in the findings table against the copy you were given. A single changed digit is a failed verification.
  6. Check the QR code's destination if you use it. A QR code on a report should resolve to a page on janoshik.com. If it points anywhere else, rely on the manual check instead.

If the codes return nothing, or the lab's copy differs from yours, the copy you hold is not the report the lab issued.

What a verified report establishes, and what it does not

Verification settles authenticity. It does not settle relevance.

A verified report establishesA verified report does not establish
The lab issued this report, with these figuresThat the tested vial came from the stated manufacturer
The sample received was analyzed on the stated dateThat your vial is from the same batch
The measured amount and purity of that sampleThat other vials in the batch match, unless variance testing was ordered
Which tests were performedAnything about tests that were not ordered
How the vial was stored before or after the analysis

Three questions connect a verified report to the material in front of you.

Is it the same lot? Match the batch or lot designation on the report with the lot number on your vial. A report on a different lot of the same compound describes different material. A certificate describes the lot that was tested and is not a claim about any other lot of the same compound.

Is it recent enough? Peptides degrade slowly even as dry powder, faster if stored warm or exposed to moisture. A report dated long before your vial was made, or on a vial kept in unknown conditions, is weaker evidence than one on fresh material.

Was the right test ordered? For identity and quantity, the standard test is informative. If your work depends on low endotoxin, absence of a second peptide, or counter-ion content, look for those specific lines.

Reading the numbers

Amount per vial. This is the measured mass of the compound in the vial, in milligrams. Compare it with the stated net content on the label. Weighing and filling small masses carries tolerance, so small deviations in either direction are normal. A large shortfall is not.

Purity. Janoshik's standard format is HPLC purity: the share of the detected related material that is the target compound. As explained in how HPLC measures peptide purity, purity is not the same as net peptide content, and a high purity figure says nothing about water or counter-ion in the powder.

Identity. The standard test confirms the named compound is present. Identity on a supplier's certificate is usually shown as a mass spectrum with an observed molecular weight, covered in how to read a peptide certificate of analysis.

Independent reports and supplier certificates

An independent report and a supplier's certificate of analysis answer different questions, and neither replaces the other.

A supplier's certificate is issued for a lot, usually covers purity and identity with the underlying chromatogram and spectrum, and sometimes net peptide content, water, counter-ion or endotoxin. An independent report is commissioned by whoever sends the sample, covers that one sample, and reports only the tests that customer paid for.

When evaluating any laboratory, including an independent one, it is reasonable to ask what method was used and whether the lab is accredited to ISO/IEC 17025, the international standard that sets requirements for the competence, impartiality and consistent operation of testing and calibration laboratories.

For Anhydrolabs material, each vial carries its lot number and is supplied as a lyophilized powder in a vacuum-sealed vial, or as 10-vial kits. The certificate of analysis for a lot is available from [email protected]; send the lot number printed on the vial. A researcher who wants an independent analysis of a lot can commission one from any laboratory. The material is for in-vitro research only, as set out in the research-use statement and in what research use only means. How a vial was stored affects what any later test will find, which is why the storage and handling page and the post on lyophilized peptides matter to anyone planning to test one.

For laboratory research use only
Every compound discussed here is supplied as a reference material for in-vitro laboratory research. Not for human or veterinary use. Nothing in this article is guidance for use in a person or an animal. See the research-use statement.

Frequently asked questions

What is Janoshik?

Janoshik Analytical is an independent analytical laboratory that tests samples sent in by customers, including research peptides, and issues a report for each one. Its public report listing states that it does not endorse, sponsor or have any affiliation with the companies or products listed.

How do I verify a Janoshik report?

Find the task number near the top of the report and the unique key at the foot. Go to janoshik.com, open the Verify page from the menu, and enter both codes. Compare the report the lab returns with your copy field by field; any difference means your copy is not the report the lab issued.

What does a standard Janoshik peptide test measure?

A standard single-peptide test covers identity, the measured amount of compound per vial in milligrams, and HPLC purity. Tests such as endotoxin, sterility, TFA content, heavy metals and LC-MS screening for peptide contamination are ordered separately and appear only if they were requested.

Does a Janoshik report prove where a sample came from?

No. The client name, sample name, manufacturer and batch on a report are details the customer supplies when ordering the test. The lab measures the vial it receives; the analysis alone cannot establish who made it or which batch it came from.

Does one report cover every vial from a supplier?

No. A report covers the single sample that was tested, or a set of vials if variance testing was ordered. It describes one lot at one point in time and is not a claim about other lots of the same compound.

Is a Janoshik report the same as a certificate of analysis?

They are related but different documents. A supplier's certificate of analysis is issued for a production lot, usually with its chromatogram and mass spectrum. An independent report is commissioned by whoever sends a sample and covers only that sample and the tests ordered.

References

  • Quality and testing

    How to Read a Peptide Certificate of Analysis

    How to read a peptide certificate of analysis: HPLC purity, mass spec identity, net peptide content, TFA vs acetate, water, endotoxin and lot numbers.

    · 10 min read

  • Quality and testing

    Peptide Purity: How HPLC Measures It

    What peptide purity means, how reversed-phase HPLC measures it at 210 to 220 nm, which impurities it catches or misses, and how it differs from content.

    · 9 min read

  • Quality and testing

    Research Peptides: What 'Research Use Only' Means

    What research peptides are, what "research use only" means under US law, how a reagent differs from a finished drug, and what a responsible supplier states.

    · 9 min read